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makin female plants produce male organs

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Old 01-13-2008, 01:27 PM   #1 (permalink)
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Default makin female plants produce male organs

this is a recipe for STS...
Originally posted by Country Mon:

First, a stock solution is made. It consists of two parts (A and B) that are initially mixed separately, then blended together. Part A is ALWAYS mixed into part B while stirring rapidly. Use distilled water; tap water may cause precipitates to form.

Wear gloves while mixing and using these chemicals, and mix and use in a properly ventilated area. A mask will prevent the breathing of any dust, which is caustic. STS is colorless and odorless, and poses minimal health risks if used as described here. (See material safety data sheet links below). Note that silver nitrate and STS can cause brown stains upon drying, so spray over newspaper and avoid spilling.

Part A: .5 gram silver nitrate stirred into 500ml distilled water
Part B: 2.5 grams sodium thiosulfate (anhydrous) stirred into 500ml distilled water

The silver nitrate dissolves within 15 seconds. The sodium thiosulfate takes 30-45 seconds to dissolve.

The silver nitrate solution (A) is then mixed into the sodium thiosulfate solution (B) while stirring rapidly. The resulting blend is stock silver thiosulfate solution (STS).

This stock solution is then diluted at a ratio of 1:9 to make a working solution. For example, 100ml of stock STS is added to 900ml of distilled water. This is then sprayed on select female plants.


The STS working solution is sprayed on select female plants until runoff. Do the spraying over newspaper in a separate area from the flower room. You probably won't smell anything, but ventilate anyway. You now have what I call a "F>M plant"; a female plant that will produce male flowers.

After the F>M plant dries move it into 12/12 immediately. This is usually done three to four weeks prior to the date that the target (to be pollinated) plants will be ready to pollinate. Response times may vary slightly depending upon the strain. More specific times can be determined by trial with your own individual strains. In my trials it took 26 days for the first pollen. 30-35 days seems optimum for planning purposes.

So, assuming that a target plant needs 3-4 weeks to produce fully mature seeds, a strain that takes 8 weeks to mature should be moved into flower at about the same time as the female>male plant. A target plant that finishes flowering in 6 weeks needs to be moved into flower later (10 days or so) so that it doesn't finish before the seeds can fully mature.

A seeded individual branch can be left to mature on a plant for a bit longer, while harvesting the other seedless buds if they finish first. Just leave enough leaves on for the plant for it to stay healthy.

Effects:

Within days I noticed a yellowing of the leaves on the F>M plants. This effect persisted for two weeks or so; after this they became green again, except for a few of the larger fans. The plants otherwise seemed healthy. No burning was observed. Growth stopped dead for the first ten days, and then resumed slowly. No stretch was ever seen. After two weeks the F>M plants were obviously forming male flower clusters. Not just a few clusters of balls, but complete male flower tops. One plant still formed some pistillate flowers, but overall it was predominantly male.

It is strange indeed to see an old girlfriend that you know like the back of your hand go through a sex change. I'll admit that things were awkward between us at first.

When the F>M plants look like they may soon open and release pollen, ( 3-1/2 to 4 weeks) move them from the main flower room into another unventilated room or closet with lighting on a 12/12 timer. Don't worry too much about watts per square foot; it will only be temporary.

When the pollen flies, move your target plants into the closet and pollinate.

A more controlled approach is to isolate the F>M plants in a third remote closet (no light is necessary in this one, as they are releasing pollen now and are nearly finished anyway). In this remote other closet the pollen is very carefully collected in a plastic produce bag or newspaper sleeve and then brought back to the lighted closet, where the target plants are now located. If this is done, be careful to not mix pollen types by letting the F>Ms dust each other. Avoid movement, or use yet another closet.

Take special care to not let pollen gather on the outside of this bag- a static charge is sometimes present. Drop small open clusters of blooms inside and then close the bag at the mouth and shake. Important: next, step outside and slowly release the excess air from the bag, collapsing it completely, so that pollen doesn't get released accidently. Point downwind; don't let it get on your hands or clothes.

This collapsed pollinated bag is now very carefully slipped over only one branch and is then tied off tightly at the mouth around the branch stem with a twist tie or tape, sealing the pollen inside. Let the bag inflate slightly with air again before sealing it off, so the branch can breathe. This technique keeps the entire plant from seeding. Agitate the bag a bit after tying it off to distribute the pollen. Don't forget to label the branch so you know which seeds are which. Other branches on this same plant can be hit with different pollen sources.

If no lighted closet is available, the plant can be moved back into the main room, but- be very careful: pollen is sneaky. After 4-5 days, the bag is gently removed and the plant completes it's flowering cycle.

Yet another method has worked well for me. I position the target plants in a non-ventilated lighted closet, and then I collect pollen on a piece of mirror or glass. This is then carefully applied to the pistils of one pre-labeled branch by using a very fine watercolor paintbrush. Care is taken to not agitate the branch or the pollen. No sneezing. The plant needs to be in place first; moving it after pollination can shake pollen free and blow this technique.

Regardless of technique, at completion you will have feminized seeds. Let them dry for 2-4 weeks
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Old 01-13-2008, 01:57 PM   #2 (permalink)
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Where would someone find these chemicals?
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Old 01-13-2008, 11:39 PM   #3 (permalink)
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here you go...
artscraft, pob 382, altamont,ny 12009
1-800-682-1730

silver nitrate and sodium thiosulfate anhydrous

.........
im sure i can find some pics of what it looks like when a succsesful turnaround occurs...if you would like...im sure most will just bypass this thread so i will leave it be for now...peace
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Old 01-14-2008, 02:05 AM   #4 (permalink)
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hmm, interesting, i wouldnt find much use for it, as im not a fan of the sausage fest garden lolol
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Old 01-14-2008, 02:07 AM   #5 (permalink)
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well to me growers like you who dont preserve genetics only provide destruction to this butifull plant....peace
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Old 01-15-2008, 05:15 PM   #6 (permalink)
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Hey 2stoned, lemme ask ya something, on this subject.
I got this feminized mazar plant, She's about 6 to 8 weeks old, although you would never know it by looking at it. Shes been through hell with me.

She's been in two different hydro experiments, and after each experiment, transplanted back into soil. She's now in soil again, resting and getting healthy.

My thoughts are, wouldn't this plant be a prime candidate to stress into growing some balls and having sex with itself? Thus producing (hopefully) some feminized mazar seeds?

So far as stressing it, what would you recommend, odd lighting hours maybe?

I'm not trying to avoid using these chemicals, but I gotta think this puppy has gotta be close to reaching it's stress limit, dont ya think?
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Old 01-15-2008, 05:42 PM   #7 (permalink)
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some plants are hard to cause herms an some are redecluslsy easy to make herm...when you say 6-8weeks in flower or in veg or total time...odd light hours will work an what i have found is that plants that are deep into flower will produce balls with light leaks in there dark cycle very easy...are you going to try to pollinate this plant with itself or save the pollen to use on another plant?...now that thats out of the way i will state my opinion on such things...i am not particluarly keen on causeing my plant stress(light, nutes, tomuch/tolittlewater) these things in my mind devolp a herm trait that you will now pass down to the next set of plants making them more herm prone to certain conditions....using the sts method this is different as you are not causine stress as you are changing the cell structre of that stem or plant...there is another method i will post that requires only silver an a couple batteries....peace
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Old 01-15-2008, 05:53 PM   #8 (permalink)
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Default improvisesed sts recipe

here
Quote:
I believe that the following is an improved procedure subsequently posted by Country Mon:

<<
Using STS For Sex Reversal: Conclusions

Hey, all...

I wanted to post some conclusions that I have come to regarding the successful reversal of plants using silver thiosulfate solution. It's been a year since I posted the other STS thread. I have done six batches myself, and have had full pollen release with all of them. Everyone else seems to be doing well, but there is very little feedback so who knows. A big thanks to those who contributed to the other thread... your input was a big help in refining the technique.

I was going to edit the original post, but I can't, as I am THE Country Mon now, not just Country Mon. Changed my email addy and got locked out.

There has been one key change that I want to pass along to everyone who didn't want to sift through the 25+ pages of the original thread.

I have discovered that using a stronger concentration of STS does not make a plant more likely to produce pollen. It just burns/stresses the plant. What DOES make a plant much more likely to complete it's mission and make pollen is a second spraying at the end of week 2.

My conclusion is that STS in any concentration is only effective at inhibiting ethylene for about 3 weeks; at that point the plant's natural female metabolism begins to take back control, and even a plant that is covered with male blooms can't finish the journey to manhood and produce pollen. A second spraying allows inhibition to last through week 6, which is more than enough time to release pollen.

Some of you have decided to use stronger concentrations of STS. This is fine as long as it doesn't burn your plants. Obviously there is a wide range of usable formulas that will work. But the second spraying is the key to follow-through. You can store the working solution you used for round one (in the spray bottle) in your refrigerator for two weeks; no need to mix a new batch from stock.

I don't see the point of going any stronger than the formula I originally came up with. It has proven itself many times over. The only change I might make is to adjust it slightly to Gobgoober's "molar-correct" mix ratio. This is not at all necessary, but does allow the most effective use of the chemicals together.

Here's a re-post of the formula mix instructions, with the adjusted recipe below that:

Preparation of STS:

First, a stock solution is made. It consists of two parts (A and B) that are initially mixed separately, then blended together. Part A is ALWAYS mixed into part B while stirring rapidly. Use distilled water; tap water may cause precipitates to form.

Wear gloves while mixing and using these chemicals, and mix and use in a properly ventilated area. A mask will prevent the breathing of any dust, which is caustic. STS is colorless and odorless, and poses minimal health risks if used as described here. (See material safety data sheet links below). Note that silver nitrate and STS can cause brown stains upon drying, so spray over newspaper and avoid spilling.

Part A: .5 gram silver nitrate stirred into 500ml distilled water
Part B: 2.5 grams sodium thiosulfate (anhydrous) stirred into 500ml distilled water

The silver nitrate dissolves within 15 seconds. The sodium thiosulfate takes 30-45 seconds to dissolve.

The silver nitrate solution (A) is then mixed into the sodium thiosulfate solution (B) while stirring rapidly. The resulting blend is stock silver thiosulfate solution (STS).

This stock solution is then diluted at a ratio of 1:9 to make a working solution. For example, 100ml of stock STS is added to 900ml of distilled water. This is then sprayed on select female plants.

Both the stock STS and the working solution should be refrigerated after use, as well as the powdered chemicals, to avoid activity loss.

The adjusted formula is as follows:

Part A: .7 gram silver nitrate stirred into 40ml distilled water
Part B: 2.6 grams sodium thiosulfate (anhydrous) stirred into 160 ml distilled water

Next, slowly add the silver nitrate solution to the sodium thiosulfate solution while stirring. This combination is then added to 800 ml of distilled water to equal 1 liter. This is your final stock solution. It is diluted 1:9 with more distilled water to make your final working solution, which then gets sprayed on your target plant.

Either formula will work great, so don't sweat it too much. But do that second spraying at the end of week 2... seems to be the key for getting pollen from the more difficult strains.

Please post your comments and experiences with STS.

CM

...

Hey there...

IndyJones... just get her nice and wet. You don't need to hit the undersides of the leaves. The exact amount isn't critical - you can really drench her and she'll be fine. She may yellow a bit, but 1:9 seems perfect. Somebody was treating individual branches, but I don't recommend this as it hormonally confuses the plant.

illkills...

The only way you will create a hermie is if you pass along hermie genes. It is not something that happens as a result of technique - you can't permanently turn a normal girl hermie (at least not by any known process), and you will NOT create herm seeds by reversing sex of the mother using STS. STS is not a stress technique; it simply inhibits a hormone (ethylene) necessary for female flower formation.

There are a number of ways a herm trait can express itself. If your plant is normally not a hermie but then herms due to chemical or light stress, it has hermie genes - they just need certain conditions to show the trait. If your plant naturally throws some bannanas towards the end of the flower cycle, it has a hermie trait, too.

In the first days of feminized seeds breeders were using light stress to create bannanas this way, which were then used as pollinators for their femmed seedlines. As a result, they carried along hermie traits to the resulting femmed seeds. So the myth was born that femmed seeds = hermie traits. Not true.

Female plants that produce an isolated few bannanas in the final couple weeks of the flower cycle are often okay to keep as clones; the bannanas come on late in the cycle so they don't usually have time to create seeds in the mom. But you have to really keep an eye on them. A few bannanas at the wrong time can really create a lot of unwanted seeds. These are not good breeders - the trait will usually show up in the offspring.

The best idea when searching for a really good female candidate to reverse is to light stress the hell out of her. You want a female that is very resistant to bannana production. This is probably your best shot at avoiding hermie traits in the resulting seeds. This is something that anyone who sells seeds should definitely do.

This isn't essential for pleasure breeders, though. If you've grown out a mom for a few generations and not seen any bannana action, give it a go.

Note that sometimes seedlings form a few bannanas along the main stem at around week 4 of 12/12, and that this is not necessarily an indication of a hermie in the genetic sense. Seedlings have higher initial gibberellin levels which allow these blooms to form. This is also why seedlings stretch so much. I have often seen this happen, with no further bannanas ever appearing in clones of the same plant.
>>

Hope it helps.

Best,

bopper
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